Fig. 1

Schematic overview and representative analyses of our multi-marker histological quantification approach based on scanned whole-slides. A Working flow of our experimental/analysis pipeline. B, C Representative quantification of cytoplasmic (B) and nuclear (C) immunohistochemical staining using single-cell segmentation performed with the HALO® software (Indica Labs, New Mexico, USA) on scanned serial sections. Left panels show whole-section scans of immunohistochemical staining. Right panels are enlarged views of the immunohistochemical staining (top), with the matching image segmentation (bottom). Nuclei are segmented in blue, and positive staining is segmented in red. Quantification results are shown in the tables associated, as follows: total number of cells (counted on the total number of nuclei on the whole scan), number of positive cells, and the percentage of positive cells over the number of total cells. Scale bar of the enlarged images = 50 µm. Abbreviations: formalin-fixed paraffin-embedded (FFPE), follicle-stimulating hormone (FSH), steroidogenic factor 1 (SF1)