Fig. 5

IL-16 expression in severe EAE spinal cord harvested 5- or 24-days post- cell or PBS injection. a Immunohistochemical images of spinal cord tissue harvested 5 days post hOM-MSC, or PBS injection stained for IL-16, CD4, CD11b and laminin. b–c. Quantification at 5 days post-hOM-MSC or PBS injection (PBS, n = 4; hOM-MSCs, n = 4). e–f. Quantification at 24 days post-hOM-MSC or PBS injection (PBS, n = 5; hOM-MSCs, n = 4). IL-16 immunofluorescence (a, shown in red) was significantly reduced in inflammatory lesions compared to PBS controls 5 days (b) and 24 days post cell injection (e). CD4 expression (a shown in green) was also reduced 5 days post cell injection (c), but this was not retained until 24 days (f). CD11b expression (a, shown in green) in hOM-MSC treated animals was significantly reduced at both 5 days (d) and 24 days (g) compared to PBS control. *p < 0.05, **p < 0.01, Students unpaired t test. Scale bar represents 50 μm