Fig. 5

Treatment with MRS5980 prevents cisplatin-induced mitochondrial dysfunction and oxidative stress in the brain. Mice were treated with cisplatin and MRS5980 (0.3 mg/kg) according to Fig. 1A. Brain tissue was collected 4 h after the last dose of cisplatin. a Synaptosomal mitochondrial function as assessed by Seahorse analysis. Maximum respiratory capacity (MRC) of mitochondria of brain synaptosomes. Data represent the increase in oxygen consumption rate (OCR) in response to addition of FCCP. Black circles: male mice, n = 5–6 and grey circles: female mice, n = 6 per group. b, c Western blot analysis of nitrosylated MnSOD in the cortex (b) and quantification of the Western blot of nitrosylated MnSOD (c). Black circles: male mice n = 4 and grey circles: female mice, n = 6 per group. Results are expressed as mean ± SEM; Two-way ANOVA with Tukey's post hoc analysis *p ≤ 0.05; **p ≤ 0.01