Fig. 4

MRS5980 preserves expression of markers of synaptic integrity in cisplatin-treated mice. Male and female mice were treated with cisplatin as in Fig. 2. Eight weeks after the last dose of cisplatin ± MRS9580 brain sections were stained for markers of synaptic integrity. a Immunofluorescence images of the pre-synaptic marker synaptophysin in the CA1 region of the hippocampus visualized by confocal microscopy, scale bar 25 µm b Quantification of synaptophysin positive puncta expressed as % of control. c Immunofluorescence images of the post-synaptic marker PSD95 in the CA1 region visualized by confocal microscopy, scale bar 25 µm. d Quantification of the PSD95 positive puncta expressed as % of control. Results are expressed as mean ± SEM; Two-way ANOVA with Tukey's post-hoc analysis ****p ≤ 0.0001. Black circles: male mice n = 7–8 and grey circles: female mice, n = 6–8 per group