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Table 1 Antibodies used for IHC and FACS

From: Brain macrophages acquire distinct transcriptomes in multiple sclerosis lesions and normal appearing white matter

Immunohistochemistry
  Company Cat # Dilution
Antibody (primary)
PLP1 (clone plpc1) AbD Serotec MCA839G 1:500
HLA-DR (clone LN3) eBioscience 14-9956 1:750
FCGR2B (datasheet CD32B) Biorbyt orb44658 1:350
ASAH1 Abcam ab74469 1:500
LGALS1    1:100
Claudin5 (clone 4C3C2) Thermo Fisher Scientific 35-2500 1:250
IBA1 Wako 019-19,741 1:1000
IBA1 Abcam ab5076 1:750
Ki67 (Clone B56) BD Pharmingen 556,003 1:400
Antibody (secondary)
Biotin-Donkey anti-Rabbit IgG JacksonImmunoResearch 711-065-152 1:300
Cy3 Goat anti-Mouse IgG JacksonImmunoResearch 115-165-003 1:200
Donkey anti-Mouse, Alexa Fluor™ 594 Thermo Fisher Scientific A21203 1:300
Donkey anti-Goat, Alexa Fluor™ 633 Thermo Fisher Scientific A21082 1:300
Streptavidin, Alexa Fluor™ 488 Thermo Fisher Scientific S11223 1:300
FACS
Antibody (primary) Company Cat # Dilution
FITC anti-human CD45 BioLegend 304,006 1:25
PE anti-human CD11b BioLegend 301,306 1:40