Fig. 8

Analysis of Aβ secretion by wild-type and S124R, R332W, N371T, S577P, R654W SORL1 CRISPR/Cas9-edited human iPSC lines. Quantification (pg/mg total protein) of Aβ40 amounts in cell-conditioned medium as determined by MSD assays. In total, Aβ secretion was measured on 8 independent WT clones, 4 SORL1-KO clones, and 3 clones for each variant (S124R, R332W, N371T, S577P, R654W). For each clone, Aβ measurement was performed in at least 2 different experiments, with 3 independent replicates in each experiment. Comparisons to WT were performed in one regression model adjusted for both clones and experiments as random effects