Fig. 6
From: RyR1-related myopathy mutations in ATP and calcium binding sites impair channel regulation
ATP binding and single channel analyses of the RyR1-RM patient-related ATP binding site mutants. A [α-32P]-ATP/ [3H]-ryanodine binding to ER microsomes of HEK293 cells expressing WT RyR1, T4979M, A538T and A538T/T4979M. B Single channel analysis of WT RyR1, T4979M, A538T and A538T/T4979M (bottom) under 10 µM Ca2+ only (left) or 10 µM Ca2+ and 1 mM ATP (right). C [3H]-ryanodine binding to ER microsomes of HEK293 cells expressing WT RyR1, T4979M, A538T and A538T/T4979M. Data are presented as mean ± S.E.M from 6 for each group