Fig. 2
From: RyR1-related myopathy mutations in ATP and calcium binding sites impair channel regulation
Structure of the ATP binding site. A The adenine ring binding site (T4979) and triphosphate tail interacting residues (K4211, K4214, and R4215) are labeled and depicted in stick representation. B [α-32P]-ATP/[3H]-ryanodine binding to ER microsomes of HEK293 cells expressing WT RyR1, T4979F, and K4211S/K4214S/R4215S. C, [3H]-ryanodine binding to ER microsomes of HEK293 cells expressing WT RyR1 and ATP binding site mutants in response to ATP. Data are presented as the mean ± S.E.M. N = 3 for each group. **P < 0.01