Fig. 2
Deposits of phosphorylated αSyn at C-terminal tyrosine residues are present in LBD brain. a Immunohistochemical staining using antibodies against pS129-αSyn ab51253, pY125-αSyn, pY133-αSyn, and pY136-αSyn in the frontal cortex from two patients with DN-LBD (case #3) and Li-LBD (case #2). Regions surrounded by white rectangles in the upper panels are magnified and shown in the lower panels. Typical pathological αSyn deposits are indicated by arrowheads. Scale bars, 50 μm. b The number of phosphorylated αSyn deposits > 2 μm2 was counted in 12 randomly selected areas of 10 mm2 in each tissue specimen using ImageJ. Data are presented as means ± standard deviation. Statistical significance was determined using the 2-tailed Student’s t test. **s < 0.01, ***P < 0.001 vs. Li-LBD