Fig. 4

Tau and p62 subcellular localization in the EC-Tau mouse dentate gyrus. a Low magnification immunohistochemistry image of the middle molecular layer (MML) of an EC-Tau mouse with low pathology (restricted to the EC): conformational abnormal human tau was labelled with antibody MC1 (red). Image shows co-labelling with an antibody against p62 (green) and DAPI labelled nuclei (blue). Scale bar represents 15 μm. b Representative immuno electron microcopy (iEM) images of synapses in the middle molecular layer (MML) of an 18 month old EC-Tau mouse double labelled with p62 (6 nm gold beads) and MC1 (10 nm gold beads). Image shows tau pathology (MC1 antibody) only. Scale bar represents 200 nm. c Representative iEM images of the colocalization of p62 (red arrow) and MC1 (blue arrow) in the middle molecular layer (MML) of an 18 month old EC-Tau mouse double labelled with p62 (6 nm gold beads) and MC1 (10 nm gold beads). Scale bar represents 200 nm.