Fig. 3

P62 accumulation precedes MC1 positive tau pathology in the EC-Tau mouse. a DAB staining of the entorhinal, perirhinal and frontal cortices on EC-Tau mice with moderate (upper panel) and severe pathology (bottom panel) for MC1 (left panel) and p62 (right panel). Scale bar represents 200 μm. b Quantification of the number of positive neurons for MC1 and p62 is shown in EC-Tau mice with mild, moderate and severe pathology for the entorhinal, perirhinal and frontal cortices. Absolute numbers, two-way ANOVA with post-hoc test. c FACS sorting of Tau biosensor HEK cells treated with brain lysate from the entorhinal (left panel) and frontal cortices (right panel) of EC-Tau mice with mild (top panel) and severe pathology (bottom panel). d Quantification of the seeding ability (% FRET in the biosensor cells) for the hippocampus and entorhinal, perirhinal and frontal cortices of EC-Tau mice with mild, moderate and severe pathology. One-way ANOVA with post-hoc test