Fig. 6

Sensory neurons overactivation promote an increase in tumor-infiltrating IL-17-producing CD4 + and CD8 + T cells. TIL from melanomas of B16F10–inoculated Nav1.8-Cre-/hM3Dq + (n = 14) and Nav1.8-Cre + /hM3Dq + (n = 13) mice were analyzed. TIL from B16F10–inoculated mice were analyzed after 4 h of culture. A Column charts show absolute numbers of CD4 + and CD8 + T cells producers of IFN-γ and IL-17. B Column charts show absolute number of γδ T cells, NKT cells and NK cells producing IFN-γ and IL-17. Cytokines levels were measured in cells isolated from tumors of B16F10–inoculated Nav1.8-Cre⁻/hM3Dq⁺ and Nav1.8-Cre⁺/hM3Dq⁺ mice. Data are shown as mean ± SEM, Unpaired t test, *.01 < P < .05; **.001 < P < .01