Fig. 5

ZNF598 regulates disease phenotypes in 5xFAD mouse model. a Enrichment of ZNF598 in the synaptosome fraction of 5xFAD mouse brain. b Immunostaining showing colocalization of ZNF598 with ribosome large subunit protein Rpl22, but not ABCE1, in MOAB2-labelled plaques (n = 50). Scale bar, 5 µm. c Immunostaining showing the number and morphology of amyloid plaques in the cortex of 5xFAD mice treated with lenti-shGFP control (n = 10) or lenti-shZNF (n = 10). Scale bar, 10 µm. d Quantification of plaque density in the hippocampus (Hip), cortex (Cort), or CA1 region of the hippocampus (CA1). e Immunostaining of sections of AD brains (n = 3) showing deposition of ZNF598, NEMF, RPL22, and RACK1, but not ABCE1, to the core of amyloid plaques (n = 55–97). Scale bar, 5 µm. Error bars, ± SEM; ^∗P < 0.05. Immunoblots represent at least 3 independent repeats