Table 1 Details of transgenic mouse models and quantification of Aβ plaque pathology. n.d.-not detected, ROI-Region of interest
Sr. No. | Transgenic mouse models | Age (months) | Sex | Genotype | No. of animals (n) | Total number of Aβ plaques/ROI stained with various antibodies | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Somatosensory cortex (SSC) | Retrosplenial cortex (RSC) | Dentate gyrus (DG) | |||||||||||||||||||
pSer8-Aβ | N3p3-Aβ | nmAβ | Aβ (4G8) | Aβ (2964) | pSer8-Aβ | N3p3-Aβ | nmAβ | Aβ (4G8) | Aβ (2964) | pSer8-Aβ | N3p3-Aβ | nmAβ | Aβ (4G8) | Aβ (2964) | |||||||
1 | 5xFAD | 5 M | Male | TREM2+/+ | 5 | Total plaque count/group | 1117 | 1664 | 1791 | 1847 | 1842 | 1353 | 1770 | 1724 | 1777 | 1815 | 933 | 1263 | 1932 | 1872 | 1857 |
Plaque load (%) | 2.89 | 8.11 | 10.91 | 11.42 | 11.46 | 4.35 | 7.49 | 7.71 | 9.27 | 8.52 | 2.89 | 5.12 | 8.76 | 8.98 | 8.93 | ||||||
TREM2−/− | 5 | Total plaque count/group | 1631 | 1836 | 2247 | 2314 | 2305 | 1734 | 1899 | 2120 | 2169 | 2198 | 1257 | 1403 | 2258 | 2196 | 2183 | ||||
Plaque load (%) | 9.53 | 10.80 | 17.90 | 19.08 | 17.49 | 7.66 | 9.29 | 13.82 | 14.41 | 14.45 | 5.27 | 6.94 | 13.55 | 14.29 | 14.22 | ||||||
15 M | Male | TREM2+/+ | 5 | Total plaque count/group | 1565 | 2322 | 3616 | 3725 | 3833 | 2057 | 2559 | 3641 | 3802 | 3778 | 1530 | 2535 | 3426 | 3537 | 3461 | ||
Plaque load (%) | 4.59 | 17.26 | 27.31 | 28.10 | 29.38 | 7.09 | 13.19 | 25.04 | 25.84 | 26.41 | 5.49 | 10.24 | 21.40 | 21.51 | 21.00 | ||||||
TREM2−/− | 5 | Total plaque count/group | 2250 | 3013 | 4414 | 4417 | 4523 | 2846 | 3285 | 4398 | 4520 | 4488 | 2153 | 3139 | 4033 | 4144 | 4096 | ||||
Plaque load (%) | 13.06 | 23.76 | 41.46 | 41.37 | 42.44 | 15.73 | 21.93 | 32.66 | 35.63 | 33.95 | 10.99 | 16.07 | 32.16 | 32.39 | 31.95 | ||||||
2 | APP/PS1L166P | 4 M | Male | TREM2+/+ | 5 | Total plaque count/group | 422 | n.d. | 1027 | 967 | 982 | 420 | n.d. | 852 | 892 | 917 | 245 | n.d. | 626 | 760 | 776 |
Plaque load (%) | 1.93 | n.d. | 5.51 | 4.96 | 5.25 | 2.46 | n.d. | 5.59 | 6.52 | 6.62 | 1.35 | n.d. | 4.14 | 5.03 | 4.63 | ||||||
TREM2−/− | 5 | Total plaque count/group | 607 | n.d. | 1215 | 1156 | 1193 | 631 | n.d. | 1062 | 1115 | 1132 | 446 | n.d. | 845 | 957 | 988 | ||||
Plaque load (%) | 2.93 | n.d. | 8.19 | 9.03 | 9.88 | 4.09 | n.d. | 7.64 | 9.14 | 9.18 | 2.46 | n.d. | 6.57 | 7.01 | 7.63 | ||||||
12 M | Female | TREM2+/+ | 5 | Total plaque count/group | 804 | 1935 | 4003 | 4090 | 3959 | 942 | 2096 | 3776 | 4050 | 3943 | 649 | 1584 | 2437 | 2482 | 2485 | ||
Plaque load (%) | 4.81 | 14.99 | 28.03 | 27.91 | 26.88 | 6.57 | 15.42 | 30.03 | 32.66 | 32.23 | 3.83 | 9.56 | 17.92 | 17.82 | 15.41 | ||||||
TREM2−/− | 5 | Total plaque count/group | 1424 | 2545 | 4655 | 4770 | 4571 | 1391 | 2562 | 4226 | 4518 | 4398 | 972 | 1951 | 2766 | 2835 | 2835 | ||||
Plaque load (%) | 9.94 | 21.24 | 39.43 | 41.24 | 40.67 | 13.65 | 21.88 | 47.34 | 47.87 | 47.04 | 7.12 | 12.86 | 24.29 | 22.81 | 25.31 | ||||||
3 | APP/PS1ΔE9 | 12 M | Female | TREM2 | 3 | Total plaque count/group | 491 | 1068 | 2044 | 2233 | 2331 | 414 | 889 | 1489 | 1785 | 1685 | 183 | 548 | 717 | 874 | 965 |
Plaque load (%) | 4.09 | 12.58 | 30.05 | 30.40 | 35.16 | 3.21 | 11.29 | 21.13 | 24.68 | 23.77 | 1.87 | 6.58 | 9.13 | 12.23 | 13.03 | ||||||
TREM2T66M | 3 | Total plaque count/group | 757 | 1397 | 2570 | 2581 | 2605 | 735 | 1349 | 1975 | 2073 | 2060 | 255 | 770 | 1001 | 1119 | 1086 | ||||
Plaque load (%) | 8.02 | 18.63 | 39.91 | 39.44 | 41.83 | 7.36 | 18.89 | 30.25 | 31.31 | 33.08 | 3.37 | 12.10 | 14.19 | 16.75 | 15.94 | ||||||