Fig. 6
Source data 5
Increased pSer8-Aβ in brain extracts of APP/PS1 ΔE9-TREM2T66M transgenic mouse brains. a–h Immunoblots and quantification showing levels of monomeric and oligomeric Aβ variants in RIPA extracts of brains of female 12 M old-APP/PS1ΔE9-TREM2T66M as compared to APP/PS1ΔE9-TREM2 mice; pSer8-Aβ immunostained with antibody 1E4E11, (monomer: t(7.993) = 2.391, *p = 0.0438; oligomer: t(7.074) = 3.925, **p = 0.0056), nmAβ detected with antibody 7H3D6 (monomer: t(7.608) = 3.849, **p = 0.0054), Aβ immunostained with antibody 4G8 (monomer: t(7.847) = 2.454, *p = 0.0402; oligomer: t(5.134) = 6.604, **p = 0.0011) or antibody 2964 (monomer: t(7.607) = 2.46, *p = 0.0408; oligomer: t(5.181) = 2.63, *p = 0.045) antibodies. i–p Immunoblots and quantification showing levels of monomeric and oligomeric Aβ variants in SDS extracts of brains of female 12 M-APP/PS1ΔE9-TREM2T66M as compared to APP/PS1ΔE9-TREM2 mice; pSer8-Aβ immunostained with antibody 1E4E11, (monomer: t(7.81) = 0.708, nsp = 0.4995; oligomer: t(5.14) = 2.62, *p = 0.0458), nmAβ detected with antibody 7H3D6 (monomer: t(7.886) = 2.449, *p = 0.0404), Aβ immunostained with antibody 4G8 (monomer: t(5.396) = 3.255, *p = 0.0202; oligomer: t(7.999) = 4.642, **p = 0.0017) or antibody 2964 (monomer: t(7.153) = 0.939, nsp = 0.3784; oligomer: t(6.775) = 2.434, *p = 0.0463) antibodies. Each dot represents ratio of Aβ signal to β-actin per animal. All data represent mean ± SEM (n = 5 animals, monomer: orange arrowheads and oligomer: blue arrowheads, color- blue (APP/PS1ΔE9-TREM2) and orange (APP/PS1ΔE9-TREM2T66M), unpaired t-test with Welch's correction). Original uncropped immunoblots are provided in the Additional file 2: