Fig. 5
TREM2T66M causes increased Aβ in the APP/PS1 transgenic mouse brains. a Representative IF images of TREM2 along with microglial marker, Iba1 in female 12 M old- APP/PS1ΔE9 and APP/PS1ΔE9-TREM2T66M mice. Dotted white boxes indicate the area shown at higher magnification (Scale bar = 50 μm, 20x). b Quantification showing decreased plaque associated Iba1+ve microglia surrounding cortical plaques in female 12 M-APP/PS1ΔE9-TREM2T66M as compared to APP/PS1ΔE9-TREM2 mice (t(2.392) = 9.566,**p = 0.0058). Each dot represents the mean value of microglia count surrounding 30 cortical plaques/animal. c Representative IF images of pSer8-Aβ and nmAβ localized in extracellular plaques, vessels (white arrowheads) and within NeuN positive neurons (yellow arrowheads) in SSC of female APP/PS1ΔE9-TREM2 and APP/PS1ΔE9-TREM2T66M mice. Dotted white boxes indicate the area shown at higher magnification (Scale bar = 50 μm, 40xW). d Quantification of intraneuronal pSer8-Aβ normalized to total number of neurons/ROI showed significantly increased intraneuronal pSer8-Aβ deposits in the SSC of female 12 M-APP/PS1ΔE9-TREM2T66M as compared to APP/PS1ΔE9-TREM2 mice (t(3.466) = 4.459, *p < 0.0154). All data represent mean ± SEM (n = 3 animals, color- blue (APP/PS1ΔE9-TREM2) and orange (APP/PS1ΔE9-TREM2T66M), unpaired t-test with Welch's correction)