Table 1 List of primary antibodies used for immunohistochemistry
Antigen | Abbreviation | Host | Dilution | Source | Catalog # (Clone) |
|---|---|---|---|---|---|
Cluster of differentiation 68 | CD68 | Rat | 1:250 | Abcam Inc., Cambridge, MA | Ab53444 (FA-11) |
Cluster of differentiation 230 | CD230 | Mouse | 1:200 | BioLegend, San Diego, CA | 808,001 (6D11) |
Complement component 3 | C3 | Rat | 1:200 | Hycult Biotech, Uden, Netherlands | HM1045 (11H9) |
Glial fibrillary acidic protein | GFAP | Rabbit | 1:2,000 | Dako/Agilent Technologies, Santa Clara, CA | Z0334 |
Ionized calcium adaptor protein 1 | Iba1 | Rabbit | 1:1,000 | Wako Chemicals Inc., Richmond, VA | 019–19,741 |
Murine Apolipoprotein E | apoE | Mouse | 1:100 | gift of D.M. Holtzman, Washington University, St. Louis, MO [57] | (HJ6.3) |
Neuronal nuclear protein | NeuN | Mouse | 1:150 | Sigma-Aldrich, St. Louis, MO | MAB377 (A60) |
Transmembrane protein 119 | TMEM119 | Rabbit | 1:350 | GeneTex, Irvine, CA | GTX134087 |
Triggering receptor expressed on myeloid cells 2 | TREM2 | Sheep | 1:150 | R&D Systems, Minneapolis, MN | AF1729 |