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Table 1 List of primary antibodies used for immunohistochemistry

From: Absence of Apolipoprotein E is associated with exacerbation of prion pathology and promotes microglial neurodegenerative phenotype

Antigen Abbreviation Host Dilution Source Catalog # (Clone)
Cluster of differentiation 68 CD68 Rat 1:250 Abcam Inc., Cambridge, MA Ab53444 (FA-11)
Cluster of differentiation 230 CD230 Mouse 1:200 BioLegend, San Diego, CA 808,001 (6D11)
Complement component 3 C3 Rat 1:200 Hycult Biotech, Uden, Netherlands HM1045 (11H9)
Glial fibrillary acidic protein GFAP Rabbit 1:2,000 Dako/Agilent Technologies, Santa Clara, CA Z0334
Ionized calcium adaptor protein 1 Iba1 Rabbit 1:1,000 Wako Chemicals Inc., Richmond, VA 019–19,741
Murine Apolipoprotein E apoE Mouse 1:100 gift of D.M. Holtzman, Washington University, St. Louis, MO [57] (HJ6.3)
Neuronal nuclear protein NeuN Mouse 1:150 Sigma-Aldrich, St. Louis, MO MAB377 (A60)
Transmembrane protein 119 TMEM119 Rabbit 1:350 GeneTex, Irvine, CA GTX134087
Triggering receptor expressed on myeloid cells 2 TREM2 Sheep 1:150 R&D Systems, Minneapolis, MN AF1729