Fig. 5

Lack of PS increases human tau phosphorylation and aggregation in the hippocampus. Biochemical analysis of phosphorylated tau in the hippocampus of WT, PS1 cKO, PS cKO, Tau, PS1 cKO;Tau and PS cKO;Tau mice at 6 months of age. Western blotting was performed using antibodies against human tau (TG5), phosphorylated tau (CP13: Ser202; PG5: Ser409), and aggregated tau (MC1). TG5 was incubated in the same membrane of CP13 blotting after stripping. The arrowhead indicates the quantified MC1-labeled high molecular weight aggregated tau species. Quantification of exogenous (human) vs endogenous (murine) phosphorylated tau levels are shown at the bottom. Phosphorylated and total tau levels are normalized to GAPDH. Values represent mean ± s.e.m. (n = 4 mice/group). Statistical analysis was determined by one-way ANOVA followed by Tukey’s post hoc tests. *P < 0.05, ** P < 0.001, *** P < 0.0001