Fig. 2

Dose-dependent presenilin effects on tau phosphorylation. Biochemical analysis showing increased tau phosphorylation in the cortex and hippocampus of PS cKO mice. Western blot images (top) and quantitative analysis (bottom) of protein lysates of cortex (a, c) and hippocampus (b, d) of WT, PS2^−/−, PS1 cKO and PS cKO mice at 9 months of age. Western blotting was performed using antibodies against total tau (Tau17025) and anti-phosphorylated tau: Ser202 (CP13), Ser202/Thr205 (AT8) and Ser396/404 (PHF-1) tau. Phosphorylated tau levels were normalized to total tau. Tau17025 was incubated in the CP13 membrane after stripping. Values represent mean ± s.e.m. (n = 4 mice/group). Statistical analysis was determined by one-way ANOVA followed by Tukey’s post hoc tests. *P < 0.05