Fig. 10

Reduced synaptic proteins and accumulation of synaptic tau in PS-deficient Tau mice. Western blot images (top) and quantification (bottom) of synaptic proteins and phosphorylated Ser202 (CP13) and total (D1M9X) tau in hippocampal lysates (left) and purified postsynaptic fractions (right) of WT, Tau, PS1 cKO;Tau and PS cKO;Tau mice at 6 months. # indicates a young 3 month-old PS cKO;Tau mouse. Protein levels were normalized to β-tubulin in the lysates and total loaded protein (1 μg) in postsynaptic fractions. D1M9X antibody was used for reblotting after CP13 membrane stripping. Notice the lack of typical presynaptic proteins (syntaxin 1A, synaptophysin, β-tubulin) in the postsynaptic fractions. Values represent mean fold ± s.e.m. (n = 3 mice/group). Statistical analysis was determined by one-way ANOVA followed by Tukey’s post hoc tests. *P < 0.05