Fig. 1

Survival times of human PrP-expressing mice (tgHu) inoculated with growth hormone CJD cases originating from France and UK. Transgenic mice that express the Met₁₂₉ (tgMet), Val₁₂₉ (tgVal) human PrP were inoculated intra-cerebrally (6 mice, 20µL per mouse) with a 10% brain homogenate from hGH-iCJD cases of French and UK origin. Two iterative passages were performed in each mouse line (Table 1). For each passage, results are presented according to the country of origin (Fr and UK) and the PRNP codon 129 genotype of patients (homozygous Met129: MM—heterozygous Met129/Val129: MV—homozygous Val129: VV). Survival time (mean ± SD in days post inoculation) in tg Met (○) and tg Val (∆). White/black symbols correspond to a type 1/type 2 PrP^res (as assessed by Western blot) in the brain of the mice respectively. Survival times in tgMet and TgVal associated with M1^CJD and V2^CJD cloned strains as well as an artificial mixture of V2^CJD + M1^CJD (10^–4 diluted) are included as reference (see Table 2)