Fig. 4

Targeted complement inhibition strongly modulates inflammatory gene expression during TBI. a Number of upregulated and downregulated genes in brains of PBS/vehicle treated (red) and CR2-Crry treated (green) mice at days 3, 7, and 28 after TBI (using NanoString Neuroinflammation panel). b Bar graph showing overall effects of CR2-Crry on gene expression at all time points after TBI. c Principal component analysis of all brain samples. d Volcano plots showing fold change and FDR of genes in PBS group (same as Fig. 1c), color coded by effects of CR2-Crry on gene expression with labeled examples. e Effect of complement inhibition on the pattern of differential expression. Table on the right shows the results of a Reactome pathway analysis of induced and inhibited genes. FDR was computed for all genes in comparisons between sham and injured brains, and for genes with p value < 0.05 in comparisons between injured groups (because of increased variability in gene expression after injury). N = 3 per group