Fig. 6

RBM5-AS1 associates with SIRT6 protein in medulloblastoma cells. A Western blot analysis of SIRT6 protein in the RNA–protein complex that was pulled down by RBM5-AS1 probes. B RIP assay performed with anti-SIRT6 antibody. The levels of RBM5-AS1 in immunoprecipitated samples were detected. *P < 0.05. C Western blot analysis showed that overexpression of RBM5-AS1 increased the protein level of SIRT6 in both DAOY and D283Med cells transfected with empty vector or RBM5-AS1-expressing plasmid. D Quantitative real-time PCR analysis of SIRT6 mRNA levels in the cells treated as in C. ns no significance. E Cycloheximide (CHX) was used to block protein synthesis, and SIRT6 protein stability was assessed