Table 1 Primary and secondary antibodies and reagents
Protein targeted | Host | Dilution | Provider | Catalogue number |
|---|---|---|---|---|
Primary antibodies | ||||
Akt | Rabbit | 1:500 | Cell Signaling | 4691S |
BLT1 | Rabbit | 1:400 | Cayman | 120114 |
ChemR23 | Mouse | 1:500 | Santa Cruz | SC-398769 |
COX-1 | Rabbit | 1:400 | R&D Systems | 4841S |
c-PLA2 | Rabbit | 1:400 | Abcam | Ab73406 |
ERK1/2 | Rabbit | 1:500 | Cell Signaling | 4696S |
FPR2 | Rabbit | 1:500 | Santa Cruz | Sc-66901 |
Galectin-3 | Goat | 1:200 | R&D Systems | AF1197 |
GFAP | Rabbit | 1:800 | Dako | Z033401-2 |
GPR18 | Rabbit | 1:400 | Sigma-Aldrich | SAB4501252 |
Iba1 | Rabbit | 1:400 | Wako | 019-19741 |
JUNK | Rabbit | 1:500 | Cell Signaling | 9252S |
LGR6 | Rabbit | 1:500 | Invitrogen | PA5-102099 |
MCSFR1 | Mouse | 1:500 | R&D Systems | MAB3291 |
PLA1 | Rabbit | 1:500 | LSBio | LS-1310189 |
p38 | Rabbit | 1:500 | Cell Signaling | 9212S |
p-Akt | Mouse | 1:400 | Cell Signaling | 12694S |
p-ERK1/2 | Mouse | 1:500 | Cell Signaling | 9106S |
p-JUNK | Mouse | 1:500 | Cell Signaling | 9255S |
p-cPLA2(S505) | Rabbit | 1:200 | Cell Signaling | 2831S |
p-p38 | Mouse | 1:200 | Cell Signaling | 9216S |
p-5-LOX | Rabbit | 1:400 | R&D Systems | pp2001 |
S100β | Mouse | 1:400 | Sigma-Aldrich | AMAB91038 |
TMEM119 | Rabbit | 1:400 | Thermo Fisher | PA5-62505 |
Trem-2 | Sheep | 1:400 | R&D Systems | AF1729 |
YKL-40 | Rat | 1:500 | R&D Systems | MAB2649 |
5-LOX | Rabbit | 1:500 | Abcam | Ab169755 |
15-LOX | Rabbit | 1:400 | Hans-Erik Claesson, KI | Ab154 |
Aβ(1–16) peptide | Mouse | 1:500 | BioLegend | 803001 |
Secondary antibodies and reagents | ||||
Alexa Fluor Plus 594 anti-mouse | Donkey | 1:400 | Invitrogen | A32744 |
Alexa Fluor Plus 488 anti-rabbit | Donkey | 1:400 | Invitrogen | A32790 |
IRDye®800CW anti-rabbit | Donkey | 1:15,000 | LiCor | 926-32213 |
IRDye®680RD anti-mouse | Donkey | 1:15,000 | LiCor | 926-68072 |
IRDye®800CW anti-rat | Goat | 1:15,000 | LiCor | 926-32219 |
IRDye®800CW anti-goat | Donkey | 1:15,000 | LiCor | 926-32214 |
Intercept® TBS Blocking buffer | LiCor | 927-66003 |