Fig. 2

Lipid droplet accumulation in perinecrotic and pseudopalisading tumor regions and corresponding GBM organoid cores. A Publicly available databases show that HILPDA is consistently increased in GBM tumors and specifically enriched in hypoxic pseudopalisading cells, which is recapitulated by the organoid core. * p < 0.01; ** p < 0.001; ns, p > 0.05. Lipid droplet staining with Oil Red O shows higher staining in the B organoid core and C pseudopalisading and perinecrotic regions of primary tumors. Scale bar for wide field images = 100 µm and 50 µm for other images. D Pie charts representing the findings in 11 patient tumors and 9 organoids