Fig. 7
Blockade of dopamine D2R did not changed the firing frequency and ISI distributions in AST-derived-neurons or mouse dopamine neuron overexpressing α-synuclein. The experiments and analyses were performed via a blinded experimental design. a1 A representative recording of a spontaneously firing AST-derived dopamine neuron before (aqua blue) and after (brown) application of sulpiride (5 μM). a2 A Representative recording of a spontaneously firing mouse dopamine neuron overexpressing α-synuclein before (navy-blue) and after (wine) application of sulpiride (5 μM). a3 Time courses of instantaneous firing frequency, action potential half-width and amplitude obtained from (a1) and (a2). b Phase-plane plot of action potentials generated from (a1). c Phase-plane plot of action potentials generated from (a2). d Interspike histogram (in ms) for four conditions, from top to bottom: baseline and during sulpiride application for AST-derived dopamine, baseline and during sulpiride application for mouse dopamine neurons overexpressing α-synuclein. n corresponds to the total number of spikes used to determine the histogram