Fig. 12
Morphological analysis after 5 months of differentiation revealed AST-derived neurons exhibit reduced arborization and larger soma area. The image analyses were performed via a blinded experimental design. a Representative images of NAS- and AST-derived neurons after 5 months of differentiation. b A schematic representation of the measurement from (a) by ImageJ. c, d Soma area and perimeter of AST-derived neurons are larger than NAS-derived neurons (Some area: t331 = -9.49, p < 0.001, two-tailed Student's t tests), (Perimeter: t331 = 14.89, p < 0.001, two-tailed Student's t tests). e Whereas, the neurite length of AST-derived neurons is shorter than NAS-derive neurons (t310 = 6.818, p < 0.001, two-tailed Student's t tests). n = 159 NAS/174 AST. Scale bar: 100 μm