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Table 1 Antibodies

From: In vivo 5-ethynyluridine (EU) labelling detects reduced transcription in Purkinje cell degeneration mouse mutants, but can itself induce neurodegeneration

Antibodies (dilution)

Source

Identifier (Cat. No; RRID)

Rabbit anti-ATF3 (1:2000)

SantaCruz

SC-188; AB_2258513

Rabbit anti-Calbindin (1:10,000)

Swant

CB-38a; AB_10000340

Rabbit anti-Cleaved caspase 3 (Asp175) (1:500)

Cell signaling technology

9661; AB_2341188

Rabbit anti-GABARAPL1 (1:1000)

Proteintech

11010-1-AP; AB_2294415

Rabbit anti-Iba1 (1:1000)

Wako

019-19741; AB_839504

Rabbit anti-IP3 receptor (1:4000)

Abcam

ab5804; AB_305124

Rabbit anti-LaminB1 (1:500)

Abcam

ab16048; AB_443298

Rat anti- LGALS3 (Mac2; 1:2000)

Cedarlane

8942AP; AB_10060357

Rabbit anti-MacroH2A1 (1:250)

Upstate biotechnology

#07–219; AB_11214187

Mouse anti-NeuN (1:2000)

Millipore

MAB377; AB_2298772

Mouse anti-Nucleoporinp62 (1:1000)

BD Pharmingen

BD 610497; AB_397863

Rabbit anti-P53 (1:2000)

Leica

P53-CM5P; AB_2744683

Mouse anti-Parvalbumin (1:1000)

Swant

235; AB_10000343

Rabbit anti-S100 (1:500)

Abcam

76729; AB_1524357

Mouse anti-p62/SQSTM1 (1:2000)

Abcam

56416; AB_945626

Mouse anti-SF3a 120 (1:500)

Synaptic systems

204 011; AB_887898

Rabbit anti-Sumo1 (1:500)

Sigma-Aldrich

SAB4503055; AB_10746310

Rabbit anti-Ubiquitin (1:2000)

Dako

Z0458; AB_2315524

Mouse anti-Ubiquitin/fk2 (1:1000)

Enzo Life Sciences

BML-PW8810-0500; AB_2051891

Mouse anti-γH2AX (1:500)

Millipore

05–636; AB_309864