Fig. 9
From: Zika virus induces neuronal and vascular degeneration in developing mouse retina
The effects of live attenuated ZIKV vaccine candidate on retina. Various dose of a live-attenuated ZIKV vaccine candidate (Vac-20 or 200 or 2000 PFU) was subcutaneously injected into neonatal mice at P0 and retinas were collected at P21. a Representative images of PI-stained retinal sections. Stars indicate retinal samples with decreased thickness. b–f Representative images of retinal flatmounts labeled with anti-CD31 for vessels, anti-Tuj1 for RGCs and their axons, anti-GFAP for astrocytes, anti-Iba1 for microglia, and anti-CD45 for leukocytes. Scale bar = 50 μm except b where scale bar = 500 μm. n = 5 for Vac-20; n = 8 for Vac-200 and Vac-2000