Fig. 5
Spinal homogenates from aged GurWT SOD1 mice seed G85R-SOD1:YFP mice inefficiently. a Scatter plot of the ages at which G85R-SOD1:YFP mice developed paralysis or were euthanized after injection of spinal homogenates. TgGurWT denotes spinal homogenate from aged GurWT mice. P1-GurWTG85R−SOD1:YFP denotes inoculum from a paralyzed first-passage G85R-SOD1:YFP animal that has been previously described [15]. The data graphed here include 3 first-passage animals described in [15]. b–d Representative images of inclusion pathology seen in cryostat sections from the spinal cord of G85R-SOD1:YFP mice that had been injected with spinal homogenates from aged GurWT mice or with homogenate from the one first-passage animal that developed paralysis (images representative of 2–3 sections per mouse). Raw data provided in Additional file 1: Data File 1. Scale bars = 20 µm