Fig. 1
From: Aβ43 aggregates exhibit enhanced prion-like seeding activity in mice
Generation of proteinase K-resistant recombinant Aβ aggregates. a Amino acid sequences of the recombinant Aβ38, Aβ40, Aβ42, and Aβ43 peptides. b Kinetics of recombinant Aβ38 (green), Aβ40 (blue), Aβ42 (black), and Aβ43 (red) aggregation in a real-time Thioflavin T (ThT) fluorescence assay. Data is mean ± s.e.m. for n = 5 independent biological replicates per Aβ variant. c Silver-stained SDS-PAGE gel of insoluble recombinant Aβ aggregates with (+) or without (−) digestion with proteinase K (PK). d Schematic of procedure used to generate PK-resistant recombinant Aβ aggregates for inoculation of AppNL−F mice. e Electron micrographs of the insoluble, PK-resistant recombinant Aβ aggregate preparations. As negative controls, sodium phosphate (NaP) buffer or non-polymerized Aβ43 were processed identically to the insoluble, PK-resistant Aβ preparations and then imaged. Scale bar = 100 nm (applies to all images)