Fig. 5
VISTA KO in microglia impairs myelin phagocytosis but not uptake of other particles. VISTA was depleted in Cx3cr1creERT2/WT VISTAloxP/loxP primary neonatal mouse microglia using 4-hydroxytamoxifen followed by incubation with pHrodo-labelled compounds. a,c,e Representative graphs of fluorescence intensity of pHrodo-labelled E. coli particles a, early apoptotic Jurkat cells c, and mouse myelin e depicted as red calibrated unit (RCU) plotted against time after addition of compounds and normalized by confluence (n = 4–5). b,d,f Fluorescence intensity normalized to confluence after 4 h incubation with pHrodo-labelled E. coli particles b, early apoptotic Jurkat cells d, and mouse myelin f. Error bars indicate mean ± s.d. (n = 4–5). Statistical analysis performed was a 2-way ANOVA with Sidak’s correction for multiple comparisons comparing KO to WT. ***p < 0.001, CytoD = Cytochalasin D, EAJ = Early apoptotic Jurkat cells