Fig. 2

VISTA KO reduces microglia ramification in PBS but not LPS-treated mice. (A) Percentage VISTA^pos microglia (DAPI^neg CD11b^pos CD45^int Ly6C^neg) in WT and VISTA KO whole brain microglia after intraperitoneal PBS or LPS injection. Error bars indicate mean ± s.d. (n = 5). Statistical analysis performed was a one-way ANOVA with Bonferroni correction for multiple comparisons. ****p < 0.0001 b Sholl analysis of WT and VISTA KO microglia after PBS (left) and LPS injection (right) (n = 25 microglia per mouse, n = 3 mice per condition). c Hierarchical clustering on principal components of 27 morphological features and corresponding genotype, condition, and cluster per cell. d Representative cell per cluster visualized as cell silhouettes. e Sholl analysis of microglia from each cluster. f Frequency of cells per cluster in WT and VISTA KO mice after PBS and LPS injection