Fig. 4
Inhibition of Aβ-induced reactive astrocytes by NLY01. a Schematic diagram showing the treatment of astrocytes with MCM from Aβ1-42 treated microglia with or without NLY01. mRNA levels of primary astrocytes 24 h post treatment with MCM from oligomeric Aβ1-42 (1 μM)-activated microglia with or without NLY01 (1 μM) is shown (n = 3 biologically independent cell cultures). b Levels of GFAP, C3, and β-actin in the hippocampus of WT and 5 × FAD mice treated with PBS or NLY01 using Western blot. c Relative GFAP and C3 protein levels were normalized versus β-actin levels (n = 4 per group). d mRNA levels of astrocyte signatures in the isolated astrocytes from the hippocampus of WT and 5 × FAD mice treated with PBS or NLY01 using qPCR. GAPDH was used to normalize for the amounts of cDNA (n = 4 per group). Data are shown as the mean \(\pm \) SEM. p values were determined by one-way ANOVA. #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001 versus Control MCM or WT + PBS; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus Aβ1-42 MCM or 5xFAD + PBS