Fig. 1

NLY01 ameliorates behavioral deficits in 5xFAD mice. 3-month-old 5xFAD mice were treated with PBS or NLY01 (1 or 10 mg/kg) subcutaneously (s.c.) for 4 months (n = 9–13 per group). a Mice were trained and tested on the spatial memory version of the Morris water maze (MWM; indicated by solid lines at 60 s escape latency) (n = 9–13 per group). b Representative swimming traces for training trials on day 5 are shown. c Mice were given a memory probe with the platform removed at 24 h after the last training trial (n = 9–13 per group). d Representative swimming traces of the memory test at 24 h after the last training trial. e Swimming speed of WT mice and 5xFAD mice treated with vehicle or NLY01 (1 or 10 mg/kg) (n = 9–13 per group). f Mice were tested on a spatial alternation task in a Y-maze (n = 11–13 per group). Data are shown as the mean \(\pm \) SEM. p values were determined by one-way ANOVA. ^#p < 0.05, ^###p < 0.001 versus WT + PBS; *p < 0.05 versus 5xFAD + PBS