Fig. 3

Basement membrane structural change in mTBI mice. a–b Representative confocal microscope images (a) and quantification (b) showing upregulation of collagen IV in mTBI-impacted ipsilateral cortical area 3 days after mTBI compare to contralateral side. Scale bar = 50 µm. c Representative confocal microscope images showing differential laminin α2 and α4 levels in mTBI-impacted ipsilateral cortical area and contralateral side 3 days after mTBI. d Quantification of α2 and α4 ratio based on fluorescent intensities in mTBI-impacted ipsilateral cortical area compare to contralateral side. Scale bar = 30 µm. n = 6 mice per group. Mean ± SD in b, d; **, P < 0.01; ***, P < 0.001; NS, non-significant (P > 0.05) by Student’s t-test