Fig. 2

Abundant neuronal αSyn inclusion pathology in the pontine nuclei of MSA patients. a Low magnification image showing abundant αSyn inclusion pathology in pontine nuclei (PN) and transverse fibers (TF) within the pons with 5H12 antibody. b, c Higher magnification showing that αSyn pathology with antibodies 5H12 (b) and 2H1 (c) in the pontine nuclei is predominantly within neuronal cell bodies (arrows) and processes (arrowheads) with a few GCIs (asterisks). d In the transverse fibers of the pons, αSyn is predominantly in GCIs (asterisks) as stained with 5H12 antibody. (e) Abundant αSyn neuroaxonal pathology (arrows) was also observed in the middle cerebellar peduncle stained with 5H12 antibody. (f) In the cerebellum, αSyn inclusions are all in the form of GCIs predominantly in the white matter (WM), but occasionally within the granular layer (GL) stained with 5H12 antibody. Neurons of the granular layer and Purkinje neurons (P) at the boundary with the  molecular layer (ML) do not have αSyn inclusion. In a, b, d–f sections were stained with 5H12 antibody. Antibody 2H1 was used in c. Sections were counterstained with hematoxylin. Scale bar = 300 μm in a, f; 120 μm in e; 60 μm in b–d