Fig. 4
PrP pancreatic expression characterization. Immunohistochemistry for PrP with (a–c) and without (d–f) proteinase K digestion in the pancreas from a subject with a normal neuropathological examination and history of T2DM (a, d), a subject with Parkinson’s disease (b, e) and with Alzheimer’s disease (c, f). Dual immunofluorescence for PrPc and glucagon (g–i), somatostatin (j–l) or insulin (m–o) to assess the cellular localization of PrP in the pancreas from a 73-year-old female with Parkinson’s disease: 40 × magnification; scale bar = 50 µm