Fig. 6
From: Enhanced detection of expanded repeat mRNA foci with hybridization chain reaction
G4C2 repeat RNAs redistribute in the nucleus during stress in C9orf72 ALS-FTD patient neurons. a R-HCR-ICC of C9orf72 ALS-FTD patient derived neurons treated with H2O or SA. G3BP1 = stress granule marker. A higher magnification of the boxed areas are shown on the right. White arrows indicate rare colocalization events between cytoplasmic G4C2 repeats and G3BP1. b Quantification of (a). Bars represent proportion of neurons with detectable G4C2 repeat RNA among total neurons ± 95%CI. N = 156 for vehicle and 535 for SA 1.0 h. c Quantification of the distribution of repeat RNA in all repeat positive neurons ± 95% CI. N = 104 for vehicle and 233 for SA 1.0 h. Statistics: Fisher’s exact test (b) and Chi-square test (c). ****p < 0.0001. Scale bar = 10 μm in (a) except for the zoomed in images (2.5 μm)