Fig. 7

Progressive loss of calbindin⁺ Purkinje cells (PC) and severely decreased expression of CALB in MBP29-hα-syn (MBP29) mice at 16 weeks of age. a Graphical overview illustrating regions of interest within the mouse cerebellum including the Purkinje cell monolayer according to [35]; coordinates: Interaural: -2.00 mm, Bregma: -5.79 mm. b Calbindin⁺ (CALB) Purkinje cells in 8- and 16-week-old mice (n = 6). Scale bar: 50 µm. c, d Quantification of Calb mRNA expression relative to the housekeepers ß-actin and 18S rRNA expressed as fold change over the mean ntg levels in 8- (c) and 16-week-old (d) MBP29-hα-syn compared to non-transgenic (ntg) mice (n = 5). e Western Blot for CALB and the housekeeper β-actin (β-act) in 8- and 16-week-old mice (n = 5). f, g Quantification of the protein level of CALB relative to ß-actin in 8- (f) and 16-week-old (g) MBP29-hα-syn compared to ntg mice. Data represent mean + standard deviation. Statistical analyses were performed using Mann–Whitney test; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001