Fig. 2

Longitudinal ECoG analysis for monitoring PTE development. a Schematic skull reproduction depicting the placement of cortical electrodes used for the ECoG recording (colored dots), ground (GND) and reference (REF) electrodes, the craniectomy (dotted circle), and the CCI injury (pink circle). b Upper row: pie charts showing the percentages of mice with PTE (blue) and without spontaneous recurrent seizures (No-SRS; orange) at the two recording periods (TBI mice, 3.5 months: n = 18; 5 months n = 29). Bottom row: bargrams depicting the number of daily SRS and their average duration in PTE mice (3.5 months: n = 8; 5 months: n = 16; one PTE mouse in cohort 1 had SRS at 3.5 months but not at 5 months). SRS were detected by 3-week (24/7) video-ECoG monitoring at each time point. Data are presented as bargrams depicting the mean ± SEM and the single values. *p < 0.05 versus 3.5 months by two tailed unpaired t-test. c, d Representative ECoG tracings of SRS recorded 5 months post-TBI in two different PTE mice. ECoG seizures may occur bilaterally (c), or unilaterally in the contralateral hemisphere (d). Black arrows indicate the beginning and end of the seizure events. e Depicts a representative ECoG tracing of a No-SRS mouse. The color code at the beginning of the traces identifies the recording electrode (see a)