Fig. 4
EFV treatment reduced PrPSc in prion infected neuronal cells. Immunoblot images of PrP signals in cell lysates of RML-infected N2a cells (a, b), RML-infected CAD5 cells (c, d) and 22L-infected CAD5 cells (e, f) treated with EFV (5 µM, 10 µM, 20 µM) or vehicle (0 µM) for three days. For PrPSc detection, samples were digested with PK (+ PK). PrP was detected using mAb 4H11. β-actin served as a loading control. The densitometric analysis of PrPSc signals (+ PK) is shown as a percentage of the signals in the untreated control cells. The data are indicated as the mean ± SEM for n = 3 per group, and the number of independent experiments = 3, each performed in triplicate. Significanc e = **p < 0.001; One-way ANOVA with Dunnett’s multiple comparison test. g Immunofluorescence images of PrPSc (Red: TRITC; Blue: DAPI) in RML-N2a cells treated with or without EFV (20 µM). The histogram represents the means ± SEM of the intensity of PrPSc staining/nuclei obtained from 3 independent experiments. Magnification: 63X. Scale bar = 20 µm. Significance = ****p < 0.0001; Student’s unpaired t test