Fig. 3
Cyp46A1 levels in prion-infected neuronal cells. a Immunofluorescence images of PrPSc (Green: Alexa Fluor 488; Red: Alexa Fluor™ 555; Blue: DAPI) for CAD5-RML and CAD5-22L. Magnification: 63X. Scale bar = 20 µm. b, c Immunofluorescence analysis of Cyp46A1 (Green: Alexa Fluor 488; Blue: DAPI) for CAD5 and CAD5-RML (Red: Alexa Fluor™ 555; Blue: DAPI) and for CAD5 and CAD5-22L cells. Fluorescence intensity was quantified; data are indicated as the mean ± SEM for n = 5 images per group from 3 independent experiments. Magnification: 63X. Scale bar = 20 µm. Significance = *p < 0.05; Student’s unpaired t test. d, e Immunoblot results of Cyp46A1 in CAD5, CAD5-RML and CAD5-22L cells. β-Actin was used as a loading control and was obtained after stripping the same membrane. The histograms depict the means ± SEM of three independent experiments. Significance = **p < 0.01; Student’s unpaired t test