Fig. 4

Thioflavin S staining in APP/PS1 mice intra-cerebrally inoculated with different AD brain specimens. Brain cortex (a–f) and hippocampus (g–l) from APP/PS1 mice injected with different human brain extracts were stained with ThS. Brains from untreated/age-matched mice (a, g) were included for comparisons. m, n Image analysis quantification (% burden) of ThS positive signals in cortex (m) and hippocampus (n) of APP/PS1 mice. o, p ThS values in each animal in cortex (o) and hippocampus (p) were divided by their corresponding Aβ values (calculated in Fig. 2) to estimate the ratio of ThS positive plaques. N = 4–6/group. Data value were expressed as mean ± SEM. Data were analyzed by one-way ANOVA followed by Tukey's multiple comparison post-test. *p < 0.05, ***p < 0.001, ****p < 0.0001