Fig. 2

Isolation of iPSN nuclei does not impact nuclear morphology. a Maximum intensity projections from SIM imaging of Lamin B1 in nuclei isolated from control and C9orf72 iPSNs at day 32 of differentiation from passage 27 iPSCs. Genotype as indicated on top. b, c Quantification of nuclear sphericity (b) and nuclear volume (c). n = 10 control and 10 C9orf72 iPSC lines, at least 50 NeuN + nuclei per line. Student’s t-test was used to calculate statistical significance. d Quantification of number of Lamin B1 invaginations per nucleus. n = 10 control and 10 C9orf72 iPSC lines, at least 50 NeuN + nuclei per line. Chi-square test was used to calculate statistical significance. Scale bar = 5 μm