Fig. 4

dlp mRNA, a Wg/Wnt interactor, is enriched in TDP-43 complexes and sequestered in insoluble fractions. a STRING diagram [102] depicting relationship between Wg/Wnt signaling genes in the top third of the TRAP control IP normalized counts (D42 > RpL10 GFP, see Materials and Methods). Line thickness between genes is correlated with the strength of the interaction as defined by STRING. b Altered ribosome association of a subset of Wnt signaling genes in TDP-43^G298S relative to control. c qPCR quantification of dlp mRNA enrichment with TDP-43 complexes in TDP-43^WT and TDP-43^G298S expressing larvae (D42 > TDP-43 WT or G298S). Enrichment in TDP-43 complexes is shown as arbitrary units (a.u.) after normalization to input. d,e. Schematic of fractionation experiments and qPCR quantification of dlp mRNA levels in the insoluble fraction (Urea fraction) of TDP-43^WT or TDP-43^G298S expressing larvae relative to w¹¹¹⁸ controls. N = (w¹¹¹⁸ = 5, other = 6) (d), and CRISPR-TDP-43^G294A (relative to CRISPR-TDP-43^WT, N = 3). Significance was determined using the Holm Sidak’s multiple comparisons test (b, d) or Student’s T Test (e). *P_value < 0.05, ***P_value < 0.001. Error bars represent SEM