Fig. 4

RN2N IgG1 and IgG2a treatments reduce total insoluble and phosphorylated tau in pR5 mice. a Schematic of RAB/RIPA brain fractionation protocol to obtain soluble (RAB) and insoluble (RIPA) fractions of whole brain lysate. b Representative images of soluble pR5 brain lysate probed with anti-tau antibodies as indicated (W = brain homogenate from wild-type mice; K = brain homogenate from MAPT knock-out mice) and quantification (n = 10 for each group). c Representative images of insoluble pR5 brain extracts probed with anti-tau antibodies as indicated and quantification (n = 10 for each group). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001