Fig. 8

Double-label fluorescent immunohistochemistry with anti-ferritin and anti-glial cell markers in nigrosome 1 in controls (Co, a–c) and patients with PD (d–f). Anti-ferritin antibody (red) in combination with anti-Olig2 antibody (green) (a, d), anti-ferritin antibody (red) combined with anti-GFAP antibody (green) (b, e) and anti-ferritin antibody (red) combined with anti-IBA-1 antibody (green) (c, f) did not show significant spatial co-localization whereas immunoreactivity in oligodendrocytes (a, d) could be confirmed. Immunohistochemical observations with anti-ferritin antibody show positive reactions in oligodendrocytes. In addition, (e) and (f) show strong activation signs of glial cells including a hypertrophic morphology and an increase in cell density. Territories of neurons surrounded by glia cell processes (a) and (d) are labelled with *. Sections were treated with Sudan black B to suppress tissue auto fluorescence. Scale bar 50 µm