Fig. 7

a–h Detection of EGFP and MCMV-E1 in neonatal mouse cerebrums infected with rMCMV1373 or rMCMV448 at 7 and 11 dpi. a–d rMCMV1373-infected cerebrums at 7 (a, b) and 11 dpi (c, d). e–h rMCMV448-infected cerebrum at 7 (e, f) and 11 dpi (g, h). In each rMCMV-infected cerebrum, fluorescence views of EGFP (a, c, e, g) and immunofluorescence for MCMV-E1 (red) (b, d, f, h) in the same field as the EGFP image are shown. Nuclei are stained with DAPI. CX, cortex; HP, hippocampus; PV, periventricular region. Scale bars: a–h 150 µm. i, j Kinetics of EGFP⁺ and MCMV-E1⁺ cells in the coronal sections of the right hemisphere of rMCMV-infected brains. Right hemispheres were divided into the PV, HP, and CX regions. The numbers of positive cells in each area at 3, 7, and 11dpi were counted and the numbers of positive cells in the hippocampal and cortical region were combined. i Kinetics of E1⁺ or EGFP⁺ cells in the PV region. j Kinetics of E1⁺ or EGFP⁺ cells in the HP and CX regions. Mean ± SEM of 3 mouse brains in each experimental group are shown (E1⁺ cells, white bar; EGFP⁺ cells, gray bar). *p < 0.01 vs. corresponding rMCMV1373-infected HP and CX in each experimental group by an unpaired 2-tailed Student’s t-test