Table 1 Primers sequences used to generate 7 PCR amplicons covering the variable regions in the bacterial 16S rRNA gene
Gene | Forward primer | Reverse primer |
|---|---|---|
V1-V2 | 5′-TCGTCGGCAGCGTCAGAGTTTGATCCTGGCTCAG-3’ | 5′-GTCTCGTGGGCTCGGTGCTGCCTCCCGTAGGAGT-3’ |
V2 | 5′-TCGTCGGCAGCGTCAGYGGCGIACGGGTGAGTAA-3’ | 5′-GTCTCGTGGGCTCGGCYIACTGCTGCCTCCCGTAG-3’ |
V3_2 | 5′-TCGTCGGCAGCGTCCCTACGGGAGGCAGCAG-3’ | 5′-GTCTCGTGGGCTCGGGTATTACCGCGGCTGCTGG-3’ |
V4 | 5′-TCGTCGGCAGCGTCGTGCCAGCMGCCGCGGTAA-3’ | 5′-GTCTCGTGGGCTCGGGGACTACHVGGGTWTCTAAT-3’ |
V5-V6 | 5′-TCGTCGGCAGCGTCAGGATTAGATACCCTGGTA-3’ | 5′-GTCTCGTGGGCTCGGCRRCACGAGCTGACGAC-3’ |
V6_1 | 5′-TCGTCGGCAGCGTCAAACTCAAAKGAATTGACGG-3’ | 5′-GTCTCGTGGGCTCGGACGAGCTGACGACARCCATG-3’ |
V7-V8 | 5′-TCGTCGGCAGCGTCGYAACGAGCGCAACCC-3’ | 5′-GTCTCGTGGGCTCGGGACGGGCGGTGWGTRC-3’ |